FACS OFF! Autonomous & High Throughput Single Cell Cloning
B-cell cloning is an industry standard method for reliable and reproducible production of monoclonal antibodies (mAb). A typical journey in mAb development involves B cells isolation prior to screening. For years, FACS has been the method of choice for semi-automated seeding of single cells into microwell plates. However, this method suffers from low throughput due to inherent difficulties to automate start up, calibration, sample/target exchange processes. Furthermore, FACS is labour-intensive as it requires continuous presence of skilled operator to monitor and troubleshoot the process. Hence, the need of an alternative approach that addresses the aforementioned shortcomings that are: throughput, clonability, and high operating costs.
In this webinar, we will present how cellenONE HT, an image-based single cell isolation platform, is fully automated using Green Button Go Scheduler so that it operates continuously without the need for an operator, effectively enabling 24/7 unattended single cell plating from one or multiple samples.
Mathieu Bennet
CTO
Cellenion
Dustin Whitson
Applications Consulting Scientist
Biosero
FAQs
Yes, the CellenONE HT is equipped with fluorescence, so you can have a usual red/green marker, which allows you to sort. So if you want to sort only live cells, you can do that.